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Performance characteristics of novel mechanical foam .

Performance characteristics of novel mechanical foam .

The resulting foam is, thus, made up of smaller bubbles which entrain a larger liquid holdup and is, consequently, more dif®cult to break. 3.5 Effect of foam-breaker clearance Foam-breaker clearance above the liquid level in the stirred tank in¯uences the ability of the foam breaker to control the rise of the foam head, as shown in Fig 10.

US20170051245A1 - Passive automatic antifoam delivery .

US20170051245A1 - Passive automatic antifoam delivery .

US20170051245A1 US14/830,306 US201514830306A US2017051245A1 US 20170051245 A1 US20170051245 A1 US 20170051245A1 US 201514830306 A US201514830306 A US 201514830306A US 2017051245 A1 US2017051245 A1 US 2017051245A1 Authority US United States Prior art keywords antifoam foam bioreactor porous object Prior art date 2015-08-19 Legal status (The legal status is an .

Nanomaterials | Free Full-Text | PEI-Mediated Transient .

Nanomaterials | Free Full-Text | PEI-Mediated Transient .

2020-8-12 · Current knowledge about recombinant protein production in insect cells at bioreactor scale is related to the BEVS, with results reported in stirred tank [4,23,24] and wave bioreactors [25,26], and high-volume shake flasks . Therefore, considering the advances reported for TGE in insect cells at small scale, there is a need to evaluate the .

Inulinolytic activity of broths of Aspergillus niger ATCC .

Inulinolytic activity of broths of Aspergillus niger ATCC .

It is the first detailed study of an inulinolytic fungus Aspergillus niger ATCC 204447 since its discovery, covering submerged cultivations both in shake flasks and a stirred tank bioreactor. Various.

Enhanced xanthan production process in shake flasks and .

Enhanced xanthan production process in shake flasks and .

2015-9-21 · mm; d t (tank diameter) = 214 mm, d i d t-1 = 0.397). The agitation speed was 200 rpm throughout the cultivation. Aeration was performed using filtered sterile air and supplied continuously to the bioreactor with rate of 1 v min-1. Foam was suppressed by the addition of silicon antifoam grade A (Sigma-Aldrich Inc., USA). During the

The Bioreactor Flashcards | Quizlet

The Bioreactor Flashcards | Quizlet

continuous stirred tank bioreactor open system where material is free to enter/exit on steady-state basis, reactants continuously added while products are continuously removed tower fermenters

Stirred tank bioreactor

Stirred tank bioreactor

Industrial Microbiology INDM 4005 Lecture 13 22/03/04

Impact of media and antifoam selection on monoclonal .

Impact of media and antifoam selection on monoclonal .

Seed train expansion. An in‐house, chimeric IgG1 producing CHO DG44 cell line was cultured in the five test media. Shake flask cultures (30 mL) were maintained in an incubator controlled at 37°C and 8% CO 2, and were agitated at a speed of 130 rpm.On day three, cells in each media were sub‐cultured into a 125‐mL single‐use spinner‐flask (Corning) containing fresh media.

Effect of antifoam agents on oxygen transfer in deep tank .

Effect of antifoam agents on oxygen transfer in deep tank .

Development of a foam breaker which operates on the principle of rapid acceleration of foam through a nozzle has allowed large‐scale aerobic fermentations to be run without addition of antifoam agents. Fermentations have been investigated over a wide range of aeration levels in which oxygen supplied to the organisms was the limiting growth factor.

Process development for scale-up production of a .

Process development for scale-up production of a .

2019-9-19 · The foam was manually controlled by adding a few drops of silicon -based antifoam (Sigma) at foam time. The temperature in the 7-L vessel was controlled at 30 °C.

Operation of a Benchtop Bioreactor | Protocol

Operation of a Benchtop Bioreactor | Protocol

Screen image of bioreactor software at the beginning of run. At the start of a stirred tank run, the temperature was set at 30 °C (yellow line), impeller speed at 200 rpm (red line), pH at 6.5 (green line), and pO 2 at 57.2% (blue line). Click here to view larger figure.

Micro-Sparger - Bioreactor replacement parts | accessories .

Micro-Sparger - Bioreactor replacement parts | accessories .

A micro sparger consists of a sparger pipe and a porous frit made of stainless steel. The application can be particularly found in the fermentation of sensitive cells and in culture media that barely foam. By using a micro sparger and compressed air the air is distributed as fine gas bubbles in the culture medium.

Antifoams | Sigma-Aldrich

Antifoams | Sigma-Aldrich

2020-8-18 · Antifoam Y-30 Emulsion (A5758 and A6457) Antifoam Y-30 Emulsion is an aqueous emulsion containing 30% active silicone. It contains non-ionic emulsifiers different from those in Antifoam Emulsions B and C. Antifoam Y-30 Emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately.

Impact of Media and Antifoam Selection on Monoclonal .

Impact of Media and Antifoam Selection on Monoclonal .

Antifoam was added to the vessels based on visual obser-vation of foam in culture vessels alone during the media scouting run, although it was noticed that dissolved oxygen measurements, shown in the top of Figure 1 for a single ves-sel, could be used as an indirect way to monitor when foam-ing occurred. This was accomplished by using a third-order

Cell Density Effects in Different Cell Culture Media and .

Cell Density Effects in Different Cell Culture Media and .

Therefore, during the bioreactor experiments, antifoam was only added to the culture dropwise and in minimal concentration (less than 3 mL for a culture volume of 2 L), only as much as necessary to dissolve the foam that was present. A single application of antifoam was sufficient to prevent foam formation during the whole experiment.

7 factors that affect oxygen transfer to cells in .

7 factors that affect oxygen transfer to cells in .

In a bioreactor, mixing is used to eliminate gradients of concentration (cell, gas, medium, and nutrient), temperature, and other properties. Mixing time is widely used to characterize mixing efficiency in a bioreactor. Mixing efficiency is one of the most significant factors affecting both performance and scale‑up in a bioreactor.

Strategies to overcome foaming and wall-growth during the .

Strategies to overcome foaming and wall-growth during the .

For continuous cell growth, the effect of inoculum age should also be considered when anti-foam is to be added. P medium strategy, without antifoam addition, not only promoted both growth (18 g l-1) and AQ production (9.8 mg g-1 DW), but also resulted in lower foaming and wall-growth (below 30% level), and higher foaming reduction (30–40%).

Performance Comparison Of CHO-S CellCultivation and mAb .

Performance Comparison Of CHO-S CellCultivation and mAb .

Level of Dissolved Oxygen . The generation of foam was reduced by adding antifoam C when required. The study was terminated after 10 days. . Application Note USD2980: Characterization and Engineering Performance of Allegro™ STR 200 Single-Use Stirred Tank Bioreactor System 2. Application Note USD3136: Characterization and Engineering .

Enhanced Natamycin production by Streptomyces natalensis .

Enhanced Natamycin production by Streptomyces natalensis .

2019-7-16 · The production process was transferred to 7.5 L-stirred tank bioreactor to investigate the possibility of scaling-up the process from shake-flask into semi-industrial bioreactor level. Results showed that maximal natamycin production was reached at the onset of the stationary phase accompanied by complete glucose exhaustion.

Bioreactor engineering for recombinant protein production .

Bioreactor engineering for recombinant protein production .

2009-8-15 · Stirred-tank bioreactor. The main advantages of stirred-tank bioreactors are their flexibility and their ability to provide high volumetric mass transfer coefficients and a homogeneous environment enabling protein production to be controlled easily. For the stirred-tank bioreactor, the impeller system (mixing system) is the most important element.

PEI-Mediated Transient Transfection of High Five Cells at .

PEI-Mediated Transient Transfection of High Five Cells at .

2020-8-12 · PEI-mediated insect cell/TGE at this level. Current knowledge about recombinant protein production in insect cells at bioreactor scale is related to the BEVS, with results reported in stirred tank [4,23,24] and wave bioreactors [25,26], and high-volume .

Strategies to overcome foaming and wall-growth during the .

Strategies to overcome foaming and wall-growth during the .

Strategies to overcome foaming and wall-growth during the cultivation of Morinda elliptica (Rubiaceae) cell suspension cultures in a stirred-tank bioreactor are described. Of all the strategies applied, only bubble-free aeration was successful in eliminating foaming by . Despite the foaming effect of around 40% in G medium strategy with 0.012% (v/v) antifoam, the maximum dry cell weight .

Efficient Production Process for Food Grade Acetic Acid .

Efficient Production Process for Food Grade Acetic Acid .

2019-7-31 · the cultivation and aeration was performed using filtered sterile air and supplied continuously to the bioreactor with rate of 0.5 v/v/min. Foam was suppressed by the addition of silicon antifoam grade A (Sigma-Aldrich Inc., MO, USA). During the cultivation process,

An Overview of Fermenter and the Design Considerations .

An Overview of Fermenter and the Design Considerations .

2014-1-8 · 7. Baffles should be provided in case of stirred fermenter to prevent vertex formation. 8. It should be provided with facility fo r intermittent addition of an antifoam agent. 9. In case of aerobic submerged fermentation, the tank should be equipped with the aerating device. 10. Provision for controlling temperature and pH of fermentation .

7 factors that affect oxygen transfer to cells in .

7 factors that affect oxygen transfer to cells in .

In a bioreactor, mixing is used to eliminate gradients of concentration (cell, gas, medium, and nutrient), temperature, and other properties. Mixing time is widely used to characterize mixing efficiency in a bioreactor. Mixing efficiency is one of the most significant factors affecting both performance and scale‑up in a bioreactor.

Continuous Processing. Performance Enhancements for .

Continuous Processing. Performance Enhancements for .

2020-8-12 · Foam sensor to automate antifoam delivery and control foam levels. . This ratio is well suited to balance oxygen demand while maintaining a sufficient level of CO 2 stripping to keep dCO 2 levels in . The literature has established empirical and theoretical models for cell culture in stirred tank reactors using oxygen transfer rates (OTRs .

Development of an Industrial Biotechnology Process .

Development of an Industrial Biotechnology Process .

2015-4-13 · The stirred tank bioreactor (STR) Basic features of a stirred tank bioreactor Oxygen delivery system – Foam control Foam control is an essential element of the operation of a sparged bioreactor. The following photograph shows the accumulation of foam in a 2 litre laboratory reactor.

Laboratory Bioreactors / Fermentors| Reactors

Laboratory Bioreactors / Fermentors| Reactors

The system consists of a compact and modular PLC-based bench-top process control system and a control panel (HMI). There are mutiple configurations available for various process sensor outputs, thermoregulation and agitator connectivity. Also, a wide range of digital/analog sensor inputs is provided, including pH, dO2, level, foam and weight.

Effects of antifoam and scale-up on operation of .

Effects of antifoam and scale-up on operation of .

1999-5-1 · The effects of antifoam on the hydrodynamics and mass transfer characteristics of the ALRs were studied using silicone-based antifoam (Sigma, USA). The surface tension was measured using a torsion balance (White Electrical, UK) with 4-cm duNouy platinum ring. Table 2 lists the different concentrations and corresponding surface tension values.

670 Albany Street 2 Tel: 617-414-2969 .

670 Albany Street 2 Tel: 617-414-2969 .

2015-12-15 · pH, dissolved oxygen (DO), temperature, agitation, foam, and level with PID control. This small-scale, stirred -tank bioreactor culture is a cost -effective method to develop new protocols and to scale up from petri dish cultures. 1. INTRODUCTION The following SOP was developed for the batch suspension culture of HEK293 F cells from Invitrogen.